Most of the work done in the lab revolves around NMR and NMR data. In general the work with NMR data requires heavy use of computers and different softwares. Some synthesis is also done in the lab, it requires less NMR, but still needs computers as well. At the moment all installations and facilities are temporary, hopefully only until the first semester of 2011, when the new building will be ready.
Several project are being realized in the lab. Since the labspace is very limited, the choice of projets is limites as well.
NMR & Statistical Analysis
The statistical analysis of NMR data (namely principal component analysis or partial least squares regression) has become fashion for mixture analyses. Without an assignment, seemingly identical mixtures can be differentiated and identified as belonging to different groups. We are using this process for the analysis of body fluids for the identification of health conditions.
Synthesis of Natural Products Skeletons
We work on the synthesis of skeletons of natural products recently published, that have shown activity against some agent of a tropical desease. We start at the skeleton, because many times that turns out to be much simpler than the whole molecule. Later derivatizations will take the skeleton closer to the natural product.
The NMR laboratory of FioCruz/FarManguinhos has 2 machines, a 400 MHz and a 500 MHz machine. Both are equipped with gradients, but only the 500 has temperatur control. Additionally the 500 has a 2.5mm probe, whereas the 400 has a HRMAS probe. Unfortunately, the HRMAS can not be used because the compressor is not working properly.